parenttriada.blogg.se - Primordia resolution

PRIMORDIA RESOLUTION PDF
PRIMORDIA RESOLUTION MANUAL

PRIMORDIA RESOLUTION MANUAL

Semi-automated segmentation requiring user input and manual labelling can be error prone.

SMC contact: cells in contact with the SMC.

CC (Companion Cell): L2 cells in apical domain in contact with the SMC with an elongated shape (as judged in ovule longitudinal median section using the “clipping plane” IMARIS tool).

resolution images, samples were stained with 50 g/mL propidium. A basal domain appears only starting stage 0-III. Separation of the site of incipient primordium formation and the meristem was carried. At stages 0-I and 0-II only an apical domain is defined.

Basal domain: group of cells at the basis of the primordium below the apical domain and until, but not including cells of the placental surface.

Apical domain: group of cells at the apex of the primordium and encompassing the SMC and direct neighbor cells.

L2 and 元 were not distinguished originally.

L1 dome: most apical cells in contact with SMC.

At stage 0-I, as enlargement is not always detected visually, the most apical L2 cell was then selected as candidate SMC (cSMC).

SMC (Spore Mother Cell): most apical central enlarged L2 cell.

Each ovule was manually labelled in Imaris using customized Cell Labels for the different cell types and domains colored as shown in Figure 1. Cell-boundary based image segmentation was done using ImarisCell (Bitplane) as described in details previously ( Mendocilla-Sato, 2017). We described previously the manipulation, staining, mounting of the flower carpels and imaging procedures ( Mendocilla-Sato, 2017). Although the story takes place after the game.

PRIMORDIA RESOLUTION PDF

Imaging of ovule primordia stained in whole-mount for cell boundary was done as described ( Mendocilla-Sato, 2017) using a laser scanning confocal microscope Leica LCS SP8 equipped with a 63X glycerol immersion objective and HyD detectors.ģD imaging and image processing (segmentation and labeling).Įntire carpels were stained using the pseudo-Schiff propidium iodide (PS-PI) cell wall staining procedure providing excellent optical transparency for 3D imaging in depth in whole-mount. illustrated PDF (Deutsche bersetzung hier traduccin espaola aqu) or ePub (smaller resolution here).